Assemble CRISPRs from metagenomic sequencing data

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

How to identify CRISPRs in sequencing data.

Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA sequences composed of a succession of repeats (23-50 bp long) separated by unique sequences called spacers. CRISPRs together with a set of genes called cas for CRISPR associated, constitute a defence mechanism against invasion by foreign sequences. We describe protocols and bioinformatics tools that allow the identifica...

متن کامل

DRISEE overestimates errors in metagenomic sequencing data

The extremely high error rates reported by Keegan et al. in 'A platform-independent method for detecting errors in metagenomic sequencing data: DRISEE' (PLoS Comput Biol 2012; 8: :e1002541) for many next-generation sequencing datasets prompted us to re-examine their results. Our analysis reveals that the presence of conserved artificial sequences, e.g. Illumina adapters, and other naturally occ...

متن کامل

OTU Analysis Using Metagenomic Shotgun Sequencing Data

Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides 16S rRNA gene fragment data with natural immunity against the biases raised during priming and thus the potential of uncovering the true struc...

متن کامل

A Massively Parallel Sequence Similarity Search for Metagenomic Sequencing Data

Sequence similarity searches have been widely used in the analyses of metagenomic sequencing data. Finding homologous sequences in a reference database enables the estimation of taxonomic and functional characteristics of each query sequence. Because current metagenomic sequencing data consist of a large number of nucleotide sequences, the time required for sequence similarity searches account ...

متن کامل

A Framework for Analysis of Metagenomic Sequencing Data

The human body is home to a diverse assemblage of microbial species. In fact, the number of microbial cells in each person is an order of magnitude greater than the number of cells that make up the body itself. Changes in the composition and relative abundance of these microbial species are highly associated with intestinal and respiratory disorders and diseases of the skin and mucus membranes....

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Bioinformatics

سال: 2016

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btw456